Journal: Animals : an Open Access Journal from MDPI
Article Title: ATR Deficiency Impairs DNA Damage Repair and Accelerates Cellular Senescence in Bovine Mammary Epithelial Cells, Leading to Lactation Dysfunction
doi: 10.3390/ani15101419
Figure Lengend Snippet: Expression of lactation-related pathway proteins in BMECs after silencing ATR gene. ( A ) Western blot image. ( B ) Grayscale analysis of mTOR protein. ( C ) Grayscale analysis of p-STAT3/STAT3 protein. ( D ) Grayscale analysis of p-STAT5/STAT5 protein. ( E ) Grayscale analysis of STAT5 protein. ( F ) Grayscale analysis of p-STAT3 protein. ( G ) Grayscale analysis of STAT3 protein. ( H ) Grayscale analysis of p-JAK2/JAK2 protein. ( I ) Grayscale analysis of p-STAT5 protein. Significant differences are indicated as * p < 0.05, *** p < 0.001, ns p > 0.05.
Article Snippet: Antibodies against p-STAT3 (1:2000, #9145), p-STAT5 (1:2000, #9359), and STAT5 (1:2000, #94205) were procured from CST (Danvers, MA, USA).
Techniques: Expressing, Western Blot